Composite

Part:BBa_K1913029:Design

Designed by: Tianhe Wang   Group: iGEM16_Wageningen_UR   (2016-10-14)


Synthetic plac-FixK2 hybrid promoter +RBS with mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 95
    Illegal NheI site found at 118
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
    Illegal AgeI site found at 717
    Illegal AgeI site found at 829
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to test synthetic plac-FixK2 hybrid promoter,we put reporter mRFP under this promoter and co-transformed with light sensor BBa_K1913034. And mRFP fluorescence value was tested so that the intensity of transcription of this promoter could be quantified.


Source

Genomic sequence of Bradyrhizobium japonicum, iGEM part registry.

References